Gel Electrophoresis - Making Agarose Gel, Protocols, and Equipment

Gel electrophoresis is the process of separating fragments of DNA and RNA by size. The most common medium for this process is agarose gel because it is inexpensive, nontoxic, and separates DNA and RNA fragments efficiently. During electrophoresis, segments of DNA or RNA are added to the agarose gel and a voltage is applied, which propels the fragments through the gel. The size of the fragments can be deduced by the distance they travel within the gel.

The gel electrophoresis process involves determining the necessary concentration of the agarose gel, making the gel, and running the gel. You’ll also need some special equipment for electrophoresis to get started.

Shop Electrophoresis Materials

If you’re ready to start running gels but need additional supplies, we’ve got you covered! You can purchase all of the electrophoresis equipment you need at RPI.

Electrophoresis Consumable Supplies:

Agarose Gel

Loading Buffer

DNA Marker/Ladder

Test Tubes

Pipet Tips


* Check out our entire selection of gloves at RPI

Electrophoresis Equipment:

Protective Glove

Electrophoresis Units & Accessories

UV Imaging System

Tube Racks

* Check out our entire selection of tube racks at RPI

Flask for Agarose Gel

Get the Complete Guide to Agarose Gel Electrophoresis

 Gel Electrophoresis - Making Agarose Gel, Protocol, and Equipment
  • Protocols for making an agarose gel
  • Protocols for running an agarose gel
  • An agarose gel concentration chart
  • Buffer solution recipes for electrophoresis
  • Electrophoresis equipment and chemical checklists