Gel electrophoresis is the process of separating fragments of DNA and RNA by size. The most common medium for this process is agarose gel because it is inexpensive, nontoxic, and separates DNA and RNA fragments efficiently. During electrophoresis, segments of DNA or RNA are added to the agarose gel and a voltage is applied, which propels the fragments through the gel. The size of the fragments can be deduced by the distance they travel within the gel.
The gel electrophoresis process involves determining the necessary concentration of the agarose gel, making the gel, and running the gel. You’ll also need some special equipment for electrophoresis to get started.
If you’re ready to start running gels but need additional supplies, we’ve got you covered! You can purchase all of the electrophoresis equipment you need at RPI.