The Mix & Go E. coli Transformation Kit and Mix & Go E. coli Transformation Buffer Set are convenient methods for the preparation of competent E. coli cells for simple and highly efficient DNA transformation. The Mix & Go method completely eliminates the requirement for heat shocking and related procedures. Instead, Mix & Go bacterial transformation can be performed by adding DNA to Mix & Go competent cells and spreading onto a plate. Transformation efficiencies are typically on the order of 108-109 transformants/µg plasmid DNA with most E. coli strains.
Uniquely formulated reagents make it easy to generate Mix & Go competent cells from current E. coli strains that are available in the laboratory. Simply grow the E. coli strain of your choice, wash, then resuspend the cells in the provided buffers. The cells are now transformation ready! The Mix & Go E. coli Transformation Kit includes all buffers and ZymoBroth medium to generate 20 ml of Mix & Go competent cells. The Mix & Go E. coli Transformation Buffer Set includes all buffers that are required to generate 60 ml of Mix & Go competent cells, and the medium (broth) is supplied by the user.
Make your own highly efficient chemically competent cells: 108- 109 transformants/µg of plasmid DNA for most common lab strains.
No heat shock or related procedures: simply add DNA and spread onto a plate -Mix & Go!
The Mix & Go E. coli Transformation Kit features a specially formulated ZymoBroth growth medium that dramatically increases E. coli transformation efficiency, typically on the order of 5 to 100-fold for most lab strains.
Product usually ships within 24 hours.
Research or further manufacturing use only, not for food or drug use.