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Tris Base Ultra Pure [Tris (Hydroxymethyl) Aminomethane], 1 Kilogram

Price$ 85.25
  • SKU: T60040-1000.0
  • Pack Size: 1 KG
Quantity
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Tris Base Ultra Pure [Tris (Hydroxymethyl) Aminomethane], 1 Kilogram

Tris (Hydroxymethyl) Aminomethane

77-86-1

121.1

C4H11NO3

Water

Room Temperature


Tris Base Ultra Pure is a commonly used reagent and buffer component. Tris Base, Tris(hydroxymethyl)aminomethane, is a primary amine that acts as a buffering agent to maintain a stable pH in experimental procedures. 

It is particularly effective in the pH range of 7.2 to 9.0, making it suitable for applications requiring slightly alkaline to mildly acidic pH conditions. Tris Base Ultra Pure is highly purified to remove impurities and contaminants, ensuring the highest quality for laboratory use. 

The ultra-pure form is essential for applications where the presence of impurities can negatively impact experimental results. Tris Base Ultra Pure is commonly used in various molecular biology techniques such as DNA and RNA extraction, protein purification, and enzymatic assays.

It is also utilized in the preparation of electrophoresis buffers and PCR reactions. 

Moisture: <0.2%
Color: <20 APHA (colorless)
Heavy Metals (as Pb): <0.0001%
Insoluble Matter: <0.005%
Heavy Metals: 5 ppm
pKa (20°C): 8.1 ± 0.1

2.5 lbs

4.50 x 6.00 x 4.50



Research or further manufacturing use only, not for food or drug use.
  • Appearance: White Crystalline Powder
  • Purity (Dried Basis By GC): ≥ 99.9 %
  • Related Substances By GC: ≤ 0.1 %
  • Water: ≤ 0.2 %
  • Loss on Drying @ 105 oC, 3hrs: ≤ 1.0 %
  • Melting Point: 170 - 172 oC
  • APHA Color, 20% Aqueous solution: ≤ 20
  • Insoluble Matter: ≤ 0.005 %
  • Heavy Metals (as Pb): ≤ 5 ppm
  • Residue on Ignition: ≤ 0.5 %
  • Iron (Fe): ≤ 5 ppm
  • UV Absorbance @260nm, 10% Aqueous solution: ≤ 0.03
  • UV Absorbance @280nm, 10% Aqueous solution: ≤ 0.02
  • UV Absorbance @290nm, 40% solution in H2O: ≤ 0.20
  • UV Absorbance @430nm, 10% Aqueous solution: ≤ 0.004
  • Identification A: Pass
  • Identification B: Pass
  • Identification C: Pass
  • pH (5% in water): 10.0 - 11.5
  • Arsenic (As): ≤ 1 ppm
  • Chloride: ≤ 100 ppm
  • Copper (Cu): ≤ 1 ppm
  • DNase, RNase, protease: None Detected
  • Lead: ≤ 1 ppm
  • Magnesium (Mg): ≤ 1 ppm
  • pH (1M in H2O): 10.5 - 11.5
  • Residual Solvents: Conforms to USP/EP
  • Residue on Ignition (USP): ≤ 0.1 %
  • Solubility (2M In H2O): Pass
  • BSE/TSE Free: BSE/TSE Free
  • Country of Origin: Report

Experiment Guides:

  • Complete Guide to Agarose Gel Electrophoresis – includes protocols for making an agarose gel, protocols for running an agarose gel, agarose gel concentration chart, buffer solution recipes for electrophoresis, and electrophoresis equipment and chemical checklists

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